Jueves, 14 de Diciembre de 2017
    MICROSCOPÍA ÓPTICA Y CONFOCAL
 

Coordinador Científico:
Fco. Javier Díez-Guerra
Responsable Técnico:
Ángeles Muñoz

 

Microscopía Óptica y Confocal

 

SMOC

 

Comunidad de Madrid

 

ÚLTIMAS NOTICIAS

  • 07-12-2017: LSM510 Vertical reparado. Se ha cambiado el escáner X y el láser de Argón (línea 488).
  • 17-11-2017: Disponible de nuevo eescáner resonante del confocal Nikon A1R.
  • 16-11-2017: Apertura   confocal LSM800 a partir del lunes 20. Para realizar las reservas y organizar las sesiones de formación y asistencia, dirigirse al SMOC (lab 310).
  • 09-02-2017 Láser Maitai del Multifotón fuera de servicio. El equipo se puede utilizar como confocal.
 

ENLACES - PROTOCOLOS - CLARIFICACIÓN DE MUESTRAS

 

  • Sistema Image-iT™ FX signal enhancer de Molecular Probes
  • Sistema FocusClear de CelExplorer
  • Optical Clearing of Biological Tissue with ScaleA2
  • Clarity resources
  • Recopilación de métodos de clarificación de muestras (Kurt Thorn)
  • McGurk, et al (2007). Three-Dimensional Imaging of Drosophila melanogaster. PloS ONE 2(9)
  • Clearing solution for deep tissue imaging: Clendenon, et al. (2011). Deep Tissue fluorescent imaging in scattering specimens using confocal microscopy. Microscopy and Microanalysis. 17, 614-617
  • Clearing solutions for deep tissue imaging: Hama, et al. (2011). Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain. Nature Neuroscience. 14, 1481-1488
  • Clearing solution for deep tissue imaging: Ertürk, et al. (2011). Three-dimensional imaging of the unsectioned adult spinal cord to assess axon regeneration and glial responses after injury. Nature Medicine. doi:10.1038/nm.2600
  • Chemical clearing and dehydratation of GFP expressing mouse brains. Becker, et al. PlosOne 2012
  • Gonzalez-Bellido PT, Wardill TJ. (2012). Labeling and confocal imaging of neurons in thick invertebrate tissue samples. Cold Spring Harb Protoc
  • Kuwajima, et al. (2013) ClearT: a detergent and solvent-free clearing method for neuronal and non-neuronal tissue. Development. 140. 1364-8
  • Chung and Deisseroth. (2013). Clarity for mapping nervous system. Nature Methods. 10. 508-513
  • Ke, et al. (2013). SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction. Nature Neuroscience
  • Luo, et al. (2013). Three-dimensional evaluation of retinal ganglion cell axon regeneration and pathfinding in whole mouse tissue after injury. Exp. Neurology. 247, 653-662
  • Chung, et al. (2013). Structural and molecular interrogation of intact biological systems. Nature. 497, 332-337
  • Meng-Tsen and Takeshi. (2014). Optical clearing of fixed brain samples using SeeDB. Curr. Protocol. Neurosci. 2.22
  • Suzaki, et al. (2014). Whole-Brain Imaging with Single-Cell Resolution Using Chemical Cocktails and Computational Analysis. Cell. 157, 726-739
  • Yang, et al. (2014). Single-Cell Phenotyping within Transparent Intact Tissue through Whole-Body Clearing. Cell. 158, 945-958
  • Renier, et al. (2014). iDISCO: A simple, rapid method to immunolabel large tissue samples for volume imaging. Cell. 159, 896-910
  • Tainaka, et al. (2014). Whole-body Imaging with Single-Cell Resolution by Tissue Decolorization. Cell. 159, 911-924
  • Belle, et al. (2014). A Simple Method for 3D Immunolabeled Axonal Tracts in Transparent Nervoues System. Cell Reports. 9, 1191-1201.
  • Epp, et al. (2015). Optimization of Clarity for Clearing Whole-Brain and Other Intact Organs. eNeuro. DOI: 10.1523/ENEURO.0022-15.2015.
  • Aoyagi, et al. (2015). A Rapid Optical Clearing Protocol Using 2,2'-Thiodiethanol for Microscopic Observation of Fixed Mouse Brain. PLOS ONE.
  • Constantini, et al. (2015). A versatile clearing agent for multimodal brain imaging. Scientifica Reports. 5. 9808.
  • Hou, et al. (2015). Scalable and DiI-compatible optical clearance of the mamalian brain. Frontiers in Neuroanatomy. Volume 9. Article 19. 
  • Richardson and Lichtman. (2015). Clarifying Tissue Clearing. Cell. 162, 246-257.
  • Hama, et al. (2015). ScaleS: an optical clearing palette for biological imaging. Nat. Neurosci. 18, 1518-1529.