Martes, 22 de Mayo de 2018

Coordinador Científico:
Fco. Javier Díez-Guerra
Responsable Técnico:
Ángeles Muñoz


Microscopía Óptica y Confocal




Comunidad de Madrid



  • 10-04-2018: Se ha cambiado el láser de Argón (líneas 488 y 514) del confocal META, vuelve a estar al 100% de su capacidad.
  • 02-01-2018: El confocal NIKON A1R+ está fuera de servicio.
  • 09-02-2017: Láser Maitai del Multifotón fuera de servicio. El equipo se puede utilizar como confocal.




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  • Padilla-Parra, et al. (2009). In The Quest Of The Best Fluorescent Protein Couple For Quantitative FRET-FLIM. Biophysical Journal. 96, 403a
  • Padilla-Parra, et al. (2009). Quantitative Comparison of Different Fluorescent Protein Couples for Fast FRET-FLIM Acquisition. Biophysical Journal. 97. 2368-2376
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  • Trembecka, et al. (2010). Conditions for using FRAP as a quantitative technique - Influence of the bleaching protocol. Cytometry. Part A. 77A, 366 - 370
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  • Charlene Depry and Jin Zhang. (2010). Visualization of Kinase Activity with FRET-Based Activity Biosensors. Current Protocols in Molecular Biology. UNIT 18.15
  • Rusanov, et al. (2010). Lifetime imaging of FRET between red fluorescent proteins. J. Biophotonics. 3, 774-783
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  • Mueller, et al. (2012). Minimizing the Impact of Photoswitching of Fluorescent Proteins on FRAP Analysis. Biophysical Journas. 102, 1656-1665.
  • Seitz, et al. (2012). Quantifying the influence of yellow fluorescent protein photoconversion on acceptor photobleaching-based fluorescence energy transfer measurements. J. Biomed. Opt. 17, 011010
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  • Kodama and Hu. (2012). Bimolecular Fluorescence Complementation (BiFC): A 5-year update and future prespectives. Biotechniques. 53, 285-298
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  • Grecco and Bastiaens. (2013). Quatifying cellular dynamics by Fluorescence Resonance Energy Transfer (FRET) microscopy. Curr. Protocol. Neurosci. 5.22.1-5.22.14
  • Kemp-O´Brien and Parsons. (2013). Using FRET to analyse signals controlling cell adhesion and migration. J. Microscopy. 251, 270-278
  • Constantini and Snapp. (2013). Probing Endoplasmic Reticulum Dynamics using Fluorescence Imaging and Photobleaching Techniques. Curr. Protoc. Cell Biol. Unit 21.7
  • Hu, et al. (2014). FRET-based and other fluorescent proteinase probes. Biotechnology J. 9, 266-281
  • Grünberg, et all. (2013). Engineering of weak helper interactions for high-efficiency FRET probes. Nature Methods. 10, 1021-1027.
  • Joosen ,et al. (2014). Effects of fixation procedures on the fluorescence lifetimesof Aequorea victoria derived fluorescent protein. J. Microscopy. 256, 166-176.
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